Site-specific analysis of drug interactions and damage in DNA using sequencing techniques
Identifieur interne : 004E14 ( Main/Exploration ); précédent : 004E13; suivant : 004E15Site-specific analysis of drug interactions and damage in DNA using sequencing techniques
Auteurs : R. J. Wilkins [Nouvelle-Zélande]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1985.
Descripteurs français
- Wicri :
- topic : ADN.
English descriptors
- KwdEn :
- Teeft :
- Acad, Academic press, Actinomycin, Adduct, Assay, Avian myeloblastosis virus, Binder, Biol, Carcinogen, Chem, Chemical cleavage, Cleavage, Coli, Coli polymerase, Control reactions, Covalent adducts, Cyclobutane pyrimidine dimers, Deoxyribonucleoside triphosphates, Dervan, Dideoxy, Dideoxynucleoside triphosphates, Dideoxyribonucleoside triphosphates, Dimer, Drug binding, Drug interactions, Drug molecules, Exact sites, Grossman, Haseltine, Inhibition, Interesting application, Klenow polymerase, Large amounts, Large number, Lesion, Many cases, Methidium propyl edta, Misincorporation, Natl, Nick translation, Nitrogen mustard, Noncovalent, Noncovalent binders, Nucl, Nucleotide, Other drugs, Other polymerases, Plasmid, Polymerase, Polymerase action, Polymerase inhibition, Practical considerations, Proc, Pulse experiments, Pydc lesions, Pyrimidine, Pyrimidine dimers, Reaction mixes, Reaction products, Residual synthesis, Restriction enzyme, Sanger, Sanger procedure, Schematic representation, Secondary structure, Secondary structures, Sequence specificities, Sequencing, Sequencing reactions, Sequencing techniques, Several weeks, Small number, Strong inhibition, Synthetic polymers, Triphosphates, Unpublished observations, Various methods, Vivo conditions, Wilkins.
Abstract
Abstract: DNA-sequencing techniques can be adapted to provide powerful analytical tools for pinpointing the sites at which DNA is modified by either radiations or chemicals. Base modifications, covalent adducts, crosslinks, and noncovalent binding can all be detected. This review outlines the adaptions of the Maxam-Gilbert and Sanger dideoxy sequencing techniques which make such studies possible. Practical aspects and limitations of the various methods are given. Assays which test the ability of DNA polymerase to bypass damage and misincorporate bases are also discussed. It is concluded that these techniques constitute the most powerful method currently available for the study of sequence-specific DNA interactions.
Url:
DOI: 10.1016/0003-2697(85)90271-4
Affiliations:
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Wilkins</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:884D58408E8FA09DE79C808DB6F05551E1C4C23C</idno><date when="1985" year="1985">1985</date><idno type="doi">10.1016/0003-2697(85)90271-4</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-9MVGQB7D-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002008</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002008</idno><idno type="wicri:Area/Istex/Curation">002008</idno><idno type="wicri:Area/Istex/Checkpoint">002281</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002281</idno><idno type="wicri:doubleKey">0003-2697:1985:Wilkins R:site:specific:analysis</idno><idno type="wicri:Area/Main/Merge">004E94</idno><idno type="wicri:Area/Main/Curation">004E14</idno><idno type="wicri:Area/Main/Exploration">004E14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Site-specific analysis of drug interactions and damage in DNA using sequencing techniques</title><author><name sortKey="Wilkins, R J" sort="Wilkins, R J" uniqKey="Wilkins R" first="R. J." last="Wilkins">R. J. Wilkins</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Molecular Carcinogenesis Laboratory, Department of Biochemistry, University of Otago Medical School, Dunedin</wicri:regionArea><wicri:noRegion>Dunedin</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Analytical Biochemistry</title><title level="j" type="abbrev">YABIO</title><idno type="ISSN">0003-2697</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">147</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="267">267</biblScope><biblScope unit="page" to="279">279</biblScope></imprint><idno type="ISSN">0003-2697</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2697</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA</term><term>damage</term><term>drugs</term><term>sequencing</term><term>site-specific</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Academic press</term><term>Actinomycin</term><term>Adduct</term><term>Assay</term><term>Avian myeloblastosis virus</term><term>Binder</term><term>Biol</term><term>Carcinogen</term><term>Chem</term><term>Chemical cleavage</term><term>Cleavage</term><term>Coli</term><term>Coli polymerase</term><term>Control reactions</term><term>Covalent adducts</term><term>Cyclobutane pyrimidine dimers</term><term>Deoxyribonucleoside triphosphates</term><term>Dervan</term><term>Dideoxy</term><term>Dideoxynucleoside triphosphates</term><term>Dideoxyribonucleoside triphosphates</term><term>Dimer</term><term>Drug binding</term><term>Drug interactions</term><term>Drug molecules</term><term>Exact sites</term><term>Grossman</term><term>Haseltine</term><term>Inhibition</term><term>Interesting application</term><term>Klenow polymerase</term><term>Large amounts</term><term>Large number</term><term>Lesion</term><term>Many cases</term><term>Methidium propyl edta</term><term>Misincorporation</term><term>Natl</term><term>Nick translation</term><term>Nitrogen mustard</term><term>Noncovalent</term><term>Noncovalent binders</term><term>Nucl</term><term>Nucleotide</term><term>Other drugs</term><term>Other polymerases</term><term>Plasmid</term><term>Polymerase</term><term>Polymerase action</term><term>Polymerase inhibition</term><term>Practical considerations</term><term>Proc</term><term>Pulse experiments</term><term>Pydc lesions</term><term>Pyrimidine</term><term>Pyrimidine dimers</term><term>Reaction mixes</term><term>Reaction products</term><term>Residual synthesis</term><term>Restriction enzyme</term><term>Sanger</term><term>Sanger procedure</term><term>Schematic representation</term><term>Secondary structure</term><term>Secondary structures</term><term>Sequence specificities</term><term>Sequencing</term><term>Sequencing reactions</term><term>Sequencing techniques</term><term>Several weeks</term><term>Small number</term><term>Strong inhibition</term><term>Synthetic polymers</term><term>Triphosphates</term><term>Unpublished observations</term><term>Various methods</term><term>Vivo conditions</term><term>Wilkins</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>ADN</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: DNA-sequencing techniques can be adapted to provide powerful analytical tools for pinpointing the sites at which DNA is modified by either radiations or chemicals. Base modifications, covalent adducts, crosslinks, and noncovalent binding can all be detected. This review outlines the adaptions of the Maxam-Gilbert and Sanger dideoxy sequencing techniques which make such studies possible. Practical aspects and limitations of the various methods are given. Assays which test the ability of DNA polymerase to bypass damage and misincorporate bases are also discussed. It is concluded that these techniques constitute the most powerful method currently available for the study of sequence-specific DNA interactions.</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li></country></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Wilkins, R J" sort="Wilkins, R J" uniqKey="Wilkins R" first="R. J." last="Wilkins">R. J. Wilkins</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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